hybridoma cell line atcc no (ATCC)

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ATCC hybridoma cell line atcc no
Hybridoma Cell Line Atcc No, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hybridoma+cells/Hybridoma+Cell+Line+12%2E1+p8/us12605469-1288-39-42
Average 90 stars, based on 22 article reviews

hybridoma cell line atcc no - by Bioz Stars, 2026-10

90/100 stars

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Incubation:

Article Title: Bemnifosbuvir and remdesivir inhibit tick-borne encephalitis virus infection in complementary in vitro and ex vivo disease models
Article Snippet: .. Plates were subsequently washed three times with 0.1% saponin or buffer composed of sodium chloride, potassium chloride, monopotassium phosphate, di-sodium hydrogen phosphate dihydrate and Tween 20 dissolved in dH 2 O (henceforth cumulatively referred to as permeabilization buffer) and subsequently incubated for up to 45 minutes at 37°C with a monoclonal pan-flavivirus anti-E protein antibody obtained from hybridoma cells (4G2, clone D1-4G2-4-15, ATCC, HB-112), diluted 1:10 in a 0.3% saponin in PBS solution. .. Following incubation, plates were washed twice with permeabilization buffer, then incubated at 37°C with a 1:250 horseradish-peroxidase conjugated secondary antibody (DAKO #P0260) in 0.3% saponin solution.

Isolation:

Article Title: Human monoclonal antibody human CD134 (OX40) and methods of making and using same
Article Snippet: .. Isolation of Human Anti-OX40 Antibody Genes: Cultured hybridoma cells (112V8 (ATCC No. PTA-7219, deposited Nov. 17, 2005)), which produce 112V8 antibody (IgG4), were collected by centrifugation. .. Total RNA was purified from these cells using RNeasy kit (QIAGEN Inc., Valencia, CA) following the manufacturer's instructions.

Cell Culture:

Centrifugation:

Transfection:

Article Title: The development of a next generation NK cell engager platform by integrating a potency-reduced IL-15 variant to enhance antitumor activity
Article Snippet: .. The supernatants of these hybridoma cells were screened against CHO-K1 cells (ATCC, catalog number CCL-61) transfected with human NKp46, while untransfected CHO-K1 cells and recombinant human IgG1 Fc were used as counter-selection controls to identify hybridomas producing NKp46-specific antibodies. .. The supernatants of these hybridoma cells were screened against CHO-K1 cells (ATCC, catalog number CCL-61) transfected with human NKp46, while untransfected CHO-K1 cells and recombinant human IgG1 Fc were used as counter-selection controls to identify hybridomas producing NKp46-specific antibodies.

Recombinant:

other:

Article Title: A novel and critical role of the intracellular Zona Pellucida protein 2 (ZP2) for blastocyst formation in mice
Article Snippet: Capped mRNA was synthesized with T7 polymerase (Ambion mMessage mMachine T7 kit) according to manufacturer’s instructions.

Purification:

Article Title: Laminin-511-functionalized fibrin gel enables in-gel proliferation of human induced pluripotent stem cells.
Article Snippet: .. The mAb TS2/16 against human integrin 1 was purified on a Protein G Sepharose 4 Fast Flow column (Cytiva, Marlborough, MA, USA; #17-0618-05) from the conditioned media of hybridoma cells obtained from the American Type Culture Collection. .. The HRP-conjugated goat pAb against rabbit IgG (#711-035-152), donkey anti-mouse IgG (#715-035-150), and Alexa FluorTM 488-conjugated donkey antigoat pAb (#705-545-147) were from Jackson ImmunoResearch Laboratories (West Grove, PA, USA).

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